The wound-healing potential of honey and propolis from stingless bees in acute wounds
Main Article Content
Keywords
acute wound, stingless bee, honey, propolis
Abstract
Introduction: Honey and propolis from stingless bees have been reported to promote wound healing due to their anti-inflammatory, antioxidant, antibacterial, and moisturizing activities. However, variations in compounds and biological activities of these products can arise due to geographical and bee origin differences. This study aimed to investigate the wound-healing potential of stingless bee honey and propolis from East Kalimantan in an acute wound animal model. Honey and propolis from stingless bees have been reported to promote wound healing due to their anti-inflammatory, antioxidant, antibacterial, and moisturizing activities. However, variations of compounds and biological activities of these products can arise due to geographical and bee origin differences. This study aimed to investigate the wound-healing potential of stingless bee honey and propolis from East Kalimantan in an acute wound animal model.
Method: A post-test-only control group design was employed in this study. Fifteen Wistar rats were divided into 3 groups, i.e., a control receiving tulle and treatment groups receiving stingless bee honey and propolis, respectively. Wound healing activity was evaluated from wound diameter changes and histological evaluations following a hole punch wound.
Result: Kruskal Wallis test results showed no significant changes in the proliferation phase of wound healing, as reflected by the diameter changes (p = 0.989), the rate of histopathological re-epithelization (p = 0.730) as well as number of fibroblasts (p = 0.779), collagen (p = 0.779), and neovascularization (p = 0.756) among the groups.
Conclusion: Honey and propolis from stingless bees have the potential to treat acute wounds in the proliferation phase, where their wound healing properties are equivalent to tulle.
References
2. Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound Healing: A Cellular Perspective. Physiol Rev. 2019;99(1):665–706. Available from: https://pubmed.ncbi.nlm.nih.gov/30475656
3. Tottoli EM, Dorati R, Genta I, Chiesa E, Pisani S, Conti B. Skin Wound Healing Process and New Emerging Technologies for Skin Wound Care and Regeneration. Pharmaceutics. 2020;12(8):735. Available from: https://pubmed.ncbi.nlm.nih.gov/32764269
4. Dhivya S, Padma VV, Santhini E. Wound dressings - a review. BioMedicine. 2015/11/28. 2015;5(4):22. Available from: https://pubmed.ncbi.nlm.nih.gov/26615539
5. Long I. Insight of the Pathophysiology of Diabetic Foot Ulcer [Internet]. Diabetic Foot - Recent Advances. IntechOpen; 2023. Available from: http://dx.doi.org/10.5772/intechopen.108190
6. Jayalakshmi MS, Thenmozhi P, Vijayaraghavan R. Plant Leaves Extract Irrigation on Wound Healing in Diabetic Foot Ulcers. Evid Based Complement Alternat Med. 2021;2021:9924725. Available from: https://pubmed.ncbi.nlm.nih.gov/34055026
7. Thomas A, Bankar N, Nagore D, Kothapalli L, Chitlange S. Herbal Oils for Treatment of Chronic and Diabetic Wounds: A Systematic Review. Curr Diabetes Rev. 2022;18(2). Available from: http://dx.doi.org/10.2174/1573399817666210322151700
8. Ramli NZ, Chin K-Y, Zarkasi KA, Ahmad F. The Beneficial Effects of Stingless Bee Honey from Heterotrigona itama against Metabolic Changes in Rats Fed with High-Carbohydrate and High-Fat Diet. Int J Environ Res Public Health. 2019;16(24):4987. Available from: https://pubmed.ncbi.nlm.nih.gov/31817937
9. Hakim SS, Wahyuningtyas RS, Siswadi S, Rahmanto B, Halwany W, Lestari F. Physicochemical Properties and Micronutrient Content of Kelulut Honey (Heterotrigona Itama) with Different Colors. Journal of Social and Economic Forestry Research. 2021;39(1):1–12.
10. Esa NEF, Ansari MNM, Razak SIA, Ismail NI, Jusoh N, Zawawi NA, et al. A Review on Recent Progress of Stingless Bee Honey and Its Hydrogel-Based Compound for Wound Care Management. Molecules. 2022;27(10):3080. Available from: https://pubmed.ncbi.nlm.nih.gov/35630557
11. Nordin A, Omar N, Sainik NQAV, Chowdhury SR, Omar E, Bin Saim A, et al. Low dose stingless bee honey increases viability of human dermal fibroblasts that could potentially promote wound healing. Wound Med. 2018;23:22–7. Available from: http://dx.doi.org/10.1016/j.wndm.2018.09.005
12. Suriawanto N, Setyawati E, Narwan. Pengaruh Pemberian Ekstrak Propolis Lebah Tanpa Sengat Pada Penyembuhan Luka Bakar Tikus Putih (Rattus norvegicus). J Bioteknol & Biosains Indones. 2021;8(1):68–76. Available from: http://dx.doi.org/10.29122/jbbi.v8i1.4585
13. Huanbutta K, Sittikijyothin W, Sangnim T. Development of topical natural based film forming system loaded propolis from stingless bees for wound healing application. J Pharm Investig. 2020;50(6):625–34. Available from: http://dx.doi.org/10.1007/s40005-020-00493-w
14. Shamsudin S, Selamat J, Sanny M, Abd. Razak S-B, Jambari NN, Mian Z, et al. Influence of origins and bee species on physicochemical, antioxidant properties and botanical discrimination of stingless bee honey. Int J Food Prop. 2019;22(1):239–64. Available from: http://dx.doi.org/10.1080/10942912.2019.1576730
15. Leonhardt SD, Rasmussen C, Schmitt T. Genes versus environment: geography and phylogenetic relationships shape the chemical profiles of stingless bees on a global scale. Proceedings Biol Sci. 2013;280(1762):20130680. Available from: https://pubmed.ncbi.nlm.nih.gov/23658202
16. Sahlan M, Rahmawati O, Pratami DK, Raffiudin R, Mukti RR, Hermasyah H. The Effects of stingless bee (Tetragonula biroi) honey on streptozotocin-induced diabetes mellitus in rats. Saudi J Biol Sci. 2019/12/02. 2020;27(8):2025–30. Available from: https://pubmed.ncbi.nlm.nih.gov/32714027
17. Masson-Meyers DS, Andrade TAM, Caetano GF, Guimaraes FR, Leite MN, Leite SN, et al. Experimental models and methods for cutaneous wound healing assessment. Int J Exp Pathol. 2020/03/30. 2020;101(1–2):21–37. Available from: https://pubmed.ncbi.nlm.nih.gov/32227524
18. Mehrabani M, Najafi M, Kamarul T, Mansouri K, Iranpour M, Nematollahi MH, et al. Deferoxamine preconditioning to restore impaired HIF-1α-mediated angiogenic mechanisms in adipose-derived stem cells from STZ-induced type 1 diabetic rats. Cell Prolif. 2015;48(5):532–49. Available from: https://pubmed.ncbi.nlm.nih.gov/26332145
19. Yudhika I, Jailani M, Dasrul. Histopathological overview of wound healing process in white rats (Rattus norvegicus) using Chromolaena odorata leaf jelly extract. J Int Surg Clin Med. 2021;1(2):21–8. Available from: http://dx.doi.org/10.51559/jiscm.v1i2.16
20. Ilenghoven DIA Review of Wound Dressing Practices. Clin Dermatology Open Access J. 2017;2(6). Available from: http://dx.doi.org/10.23880/cdoaj-16000133
21. Mama M, Teshome T, Detamo J. Antibacterial Activity of Honey against Methicillin-Resistant Staphylococcus aureus: A Laboratory-Based Experimental Study. Int J Microbiol. 2019;2019:7686130. Available from: https://pubmed.ncbi.nlm.nih.gov/31073310
22. Sarheed O, Ahmed A, Shouqair D, Boateng J. Antimicrobial Dressings for Improving Wound Healing [Internet]. Wound Healing - New insights into Ancient Challenges. InTech; 2016. Available from: http://dx.doi.org/10.5772/63961
23. Han G, Ceilley R. Chronic Wound Healing: A Review of Current Management and Treatments. Adv Ther. 2017/01/21. 2017;34(3):599–610. Available from: https://pubmed.ncbi.nlm.nih.gov/28108895
24. Al-Hatamleh MAI, Boer JC, Wilson KL, Plebanski M, Mohamud R, Mustafa MZ. Antioxidant-Based Medicinal Properties of Stingless Bee Products: Recent Progress and Future Directions. Biomolecules. 2020;10(6):923. Available from: https://pubmed.ncbi.nlm.nih.gov/32570769
25. Tashkandi H. Honey in wound healing: An updated review. Open life Sci. 2021;16(1):1091–100. Available from: https://pubmed.ncbi.nlm.nih.gov/34708153
26. Syafrizal, Ramadhan R, Wijaya Kusuma I, Egra S, Shimizu K, Kanzaki M, et al. Diversity and honey properties of stingless bees from meliponiculture in East and North Kalimantan, Indonesia. Biodiversitas J Biol Divers. 2020;21(10). Available from: http://dx.doi.org/10.13057/biodiv/d211021
27. Yanti EN, Kustiawan PM. Study of Indonesian Stingless Bee Propolis Potential As Antioxidant: A Review. J Farm Sains dan Prakt. 2023;261–9. Available from: http://dx.doi.org/10.31603/pharmacy.v9i3.7105
28. Suvik A, Effendy AWM. The use of modified Masson’s trichrome staining in collagen evaluation in wound healing study. Mal J Vet Res. 2012;3(1):39–47.
29. Darby IA, Hewitson TD. Fibroblast Differentiation in Wound Healing and Fibrosis [Internet]. International Review of Cytology. Elsevier; 2007. p. 143–79. Available from: http://dx.doi.org/10.1016/s0074-7696(07)57004-x
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
Viky Hibatu Wafi, General Surgery Residency Program, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
General Surgery Residency Program, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
Abdul Azis Boenjamin, General Surgery Residency Program, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
General Surgery Residency Program, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
Ivan Joalsen Mangara Tua, Thoracic, Cardiac and Vascular Surgery Division, Abdul Wahab Sjahranie General Hospital, Samarinda, Indonesia
Thoracic, Cardiac and Vascular Surgery Division, Abdul Wahab Sjahranie General Hospital, Samarinda, Indonesia
Swandari Paramita, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
Endang Sawitri, Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
Faculty of Medicine, Universitas Mulawarman, Samarinda, Indonesia
Yudhy Arius, Plastic Surgery Division, Abdul Wahab Sjahranie General Hospital, Samarinda, Indonesia
Plastic Surgery Division, Abdul Wahab Sjahranie General Hospital, Samarinda, Indonesia